Includes a title page in Arabic

Thesis (M.Sc.) Master Egypt - Japan University of Science and Technology (E - JUST) - School of the Institute of Basic and Applied Sciences - Biotechnology Department 2024

Includes bibliographical references

Lignocellulose is regarded as one of the most essential recalcitrant materials to be degrade attributed to its high complexity Fungi have been used in the bioremediation of different organic substances due to production of different intra and extracellular specific enzymes In this context , white rote fungus produces unique enzymes involved in lignin degradation including , manganese peroxidase (MnP) , lignin peroxidase (LiP) , and laccase (Lac) Herein , we evaluate the ligninolytic activity of two fungal isolates Aspergillus neoindicus OR378285 and Pleurostoma richardsiae PP763383 using solid-state fermentation (SSF) onto rice straw (RS) , date palm leaves (DPL) and rice husk (RH) raw materials Chemical compositions of lignocellulose biomass were determined In addition , the potential lignocellulosic , fermentation conditions and ligninolytic enzymes activity and purification were characterized The fermentation conditions including , humidity content, temperature, pH, and salt concentration were optimized to maximize ligninolytic enzymes production during 7, 14 and 21 days As a result, the maximum enzyme activity of Lac and MnP of Aspergillus neoindicus onto RS, DPLs and RH were, 921.2±8.0, 941.2±8.1, 822.2±7.4, 862.3.3±7.6, 642.3±3.1 and 723.3±5.1 U/ml respectively While the maximum enzyme activity of Pleurostoma richardsiae were ,643.4.2, 812.3±7.1, 734.2± 6.078, 748.45 ± 4.53 U/ml, 643.2±3.3 and 723.3±5.1 U/ml on RS, DPLs and RH were observed at 30°C, pH 5.0, 8% water content and 8% inoculum size, respectively after 14 days However, fermentation for 7, 14 and 21 days at 25°C, 33 °C and 37°C displayed lower Lac and MnP activity Our results indicated that CuSO4 at 7.83 µM, MgSO4 25.9 µM, MnSO4 20.6 µM, FeSO4 6.25 µM, ZnSO4 3.8 µM and CaCl2 5.6 µM were the ideal salt concentrations Metal ions including CuSO4, MgSO4, MnSO4, FeSO4, ZnSO4 and CaCl2 enhances the fungal enzymes involved into delignification. Enzyme purification results showed that Lac and MnP produced by both fungal strains having molecular weight of approximately 67 kDa and 38 KDa, respectively Chemical compositions using technical association of the pulp and paper industry analysis were also determined As results, the chemical compositions of extractives , cellulose , lignin , and hemicellulose of the DPLs fermented with Aspergillus neoindicus were 18.0%, 24.3%, 8.3%, 7.3%, 8.4%, 29.3%, 19.4%, and 14.2%, 6.7%, 19.2%

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(BAS) Institute of Basic and Applied Science (BIO) Biotechnology