000 07104nam a2200457 4500
003 EG-NbEJU
005 20250220144354.0
008 250220b2025 ua d | m||| 00| 0 eng d
040 _aEG-NbEJU
_beng
_cEG-NbEJU
_dEG-NbEJU
041 _aeng
_bara
050 _aEPE PhD. 2025
_b 01
100 1 _aSaleh , Abdelatty Mohamed Abdelatty ,
245 1 0 _aDetection of Bacterial Pathogen(s) in Freshwater Fish using Microbiological and Single Chain Fragment Antigen-Binding Region Techniques :
_bA Thesis Submitted to the Graduate School of Basic and Applied Science : Egypt-Japan University of Science and Technology (E-JUST) : In Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy in Biotechnology /
_cby Abdelatty Mohamed Abdelatty Saleh ; Supervisor Committee Prof. Ahmed Lotfy Abdel-Mawgood - Biotechnology Program, Institute of Basic and Applied Science, Egypt- Japan University of Science and Technology , Prof. Mohamed Abd El-Hady Ghazy - Biotechnology Program, Institute of Basic and Applied Science, Egypt- Japan University of Science and Technology , Prof. Alaa Eldin Abdel Mouty Mohamed Eissa - Department of Aquatic Animal Medicine and Management - Faculty of Veterinary Medicine - Cairo University , Associate Prof. Sarah Osama Saad El-Sayed - Oceanography Department - Faculty of Science - Alexandria University ; Examination Committee Prof. Ahmed Lotfy Abdel- Mawgood - Institute of Basic and Applied Science, Egypt-Japan University of Science and Technology , Prof. Mohamed Abd El-Hady Ghazy - Institute of Basic and Applied Science, Egypt-Japan University of Science and Technology , Prof. Magdy Khalil Soliman - Faculty of Veterinary Meditie - Damanhour University , Prof. Alkhateib Yousry Gaafar - Veterinary Research Institute - National Research Centre
246 1 5 _aالكشف عن أمراض بكتيرية في أسماك المياه العذبة باستخدام تقنيات الميكروبيولوجيا و الأجسام المضادة وحيدة السلسلة :
_bرسالة علمية مقدمة إلى المدرسة التخصصية للدرسات العليا العلوم الأساسية و التطبيقية : الجامعة المصرية اليابانية للعلوم و التكنولوجيا كاستيفاء جزئي لمتطلبات الحصول على درجة دكتوراه الفلسفة في البيوتكنولوجي / إعداد عبدالعاطي محمد عبدالعاطی صالح ; لجنة الإشراف على الرسالة أ . د أحمد لطفي عبدالموجود , أ . د محمد عبدالهادي غازی , أ . د علاء الدين عبد المعطي محمد عیسی , أ . م . د ساره اسامه سعد السيد ; لجنة المناقشة و الحكم على الرسالة أ . د أحمد لطفي عبدالموجود , أ . د محمد عبدالهادي غازی , ا . د مجدى خليل سلیمان , ا . د الخطيب يسرى جعفر
260 _aAlexandria :
_bAbdelatty Mohamed Abdelatty Saleh
_c2025
300 _a109 leaves :
_btable ;
_c30 cm
500 _aIncludes a title page in Arabic
502 _aThesis (Ph.D.)
_bDoctoral
_cEgypt - Japan University of Science and Technology (E-JUST) - School of Basic and Applied Science - Biotechnology Department
_d2025
520 _aFish are a valuable food source due to their high nutritional value, palatability, and digestibility However, diseases exacerbated by intensive fish farming pose significan challenges High-density farming and pe margement often lead to chronic stres immension, and water commination, promoting bacterial outbreaks Key pathogens, such as Aeromonas hydrophila and Streptococcus agalactie, thrive in these environments, causing substantial losses in fish populations and potential threats to hum health Traditional methods of detecting bacterial pathogens, like culture-based techniques, are time- consuming and inadequate for identifying non-cultivable bacteria. Advanced techniques, such as 16S rRNA gene sequencing and immunoassays, erable more accurate identification and monitoring of pathogens The aquatic environment represents a potential source for transmitting A. hydrophila, which increase the mass mortalities in fish farms. This work aimed to determine the prevalence of A hydrophila in Ö. niloticus farms. Random samples, including water, sediment, and fish, were collected A total of 45 A. hydrophila isolates were presumptively identified using morph-chemical conventional tests A total of 42 A. Apdrophila isolates proved to be lipase producers Staphylococcus epidermidis has been identified as a causative agent of mass mortality in farmed O.milovics in Kafrelsheikh Governorate The collected diseased fish exhibited sever lesions in the hepatopancreas and eyes Isolates showed Gram-positive cocci characteristics and went unikt, congelamengutive, and murtutumdyä, un sirup likod ngan Tundu identification via 16S rRNA sequencing confirmed them as S. epidermidis. The four isolates in this study have similarities to other S. epidermidis isolates as follows : ASI strain of S epidermidis has a nucleotide sequence of 99.63%, similar to the reference isolate ; S. epidermidis sirain B7.Found in Shidal, India, A52 strain is 99.77% identical to the reference strain, LGC 305, which was isolated from the gut of a freshwater Indian loch ; while AS3 and AS4 strains are 99.86% and 99.99% identical to the reference strain found in Chinese fermented fish, respectively All eight isolates were subjected to an antibiogram test, and they were resistant to ampicillin, erythromycin, chloramphenicol, streptomycin, and amoxicillin however, they were slightly susceptible to tetracycline and were sensitive to gentamicin and vancomycin
530 _aIssued also as a digital file (for more information please check our Digital Repository)
590 _aAnD
_bACM
700 1 _aAbdel-Mawgood , Ahmed Lotfy ,
_eSupervisor
700 1 _aGhazy , Mohamed Abd El-Hady ,
_eco-Supervisor
700 1 _aEissa , Alaa Eldin Abdel Mouty Mohamed ,
_eco-Supervisor
700 1 _aEl-Sayed , Sarah Osama Saad ,
_eco-Supervisor
700 1 _aAbdel-Mawgood , Ahmed Lotfy ,
_eExaminator
700 1 _aGhazy , Mohamed Abd El-Hady ,
_eco-Examinator
700 1 _aSoliman , Magdy Khalil ,
_eco-Examinator
700 1 _aGaafar , Alkhateib Yousry ,
_eco-Examinator
700 1 _aعبدالموجود , أحمد لطفي ,
_eمشرف
700 1 _aغازی , محمد عبدالهادي ,
_eمشرف مساعد
700 1 _aعیسی , علاء الدين عبد المعطي محمد ,
_eمشرف مساعد
700 1 _aالسيد , ساره اسامه سعد ,
_eمشرف مساعد
700 1 _aعبدالموجود , أحمد لطفي ,
_eمناقش
700 1 _aغازی , محمد عبدالهادي ,
_eمناقش مساعد
700 1 _aسلیمان , مجدى خليل ,
_eمناقش مساعد
700 1 _aجعفر , الخطيب يسرى ,
_eمناقش مساعد
901 _aHaGeR
902 _aEJUST_1302_ (2)
942 _2lcc
_n0
_cDISS
999 _c7160
_d7160