Detection of Bacterial Pathogen(s) in Freshwater Fish using Microbiological and Single Chain Fragment Antigen-Binding Region Techniques : (Record no. 7160)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 07104nam a2200457 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-NbEJU |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250220144354.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250220b2025 ua d | m||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-NbEJU |
| Language of cataloging | eng |
| Transcribing agency | EG-NbEJU |
| Modifying agency | EG-NbEJU |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| Language code of summary or abstract | ara |
| 050 ## - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | EPE PhD. 2025 |
| Item number | 01 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Saleh , Abdelatty Mohamed Abdelatty , |
| 245 10 - TITLE STATEMENT | |
| Title | Detection of Bacterial Pathogen(s) in Freshwater Fish using Microbiological and Single Chain Fragment Antigen-Binding Region Techniques : |
| Remainder of title | A Thesis Submitted to the Graduate School of Basic and Applied Science : Egypt-Japan University of Science and Technology (E-JUST) : In Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy in Biotechnology / |
| Statement of responsibility, etc. | by Abdelatty Mohamed Abdelatty Saleh ; Supervisor Committee Prof. Ahmed Lotfy Abdel-Mawgood - Biotechnology Program, Institute of Basic and Applied Science, Egypt- Japan University of Science and Technology , Prof. Mohamed Abd El-Hady Ghazy - Biotechnology Program, Institute of Basic and Applied Science, Egypt- Japan University of Science and Technology , Prof. Alaa Eldin Abdel Mouty Mohamed Eissa - Department of Aquatic Animal Medicine and Management - Faculty of Veterinary Medicine - Cairo University , Associate Prof. Sarah Osama Saad El-Sayed - Oceanography Department - Faculty of Science - Alexandria University ; Examination Committee Prof. Ahmed Lotfy Abdel- Mawgood - Institute of Basic and Applied Science, Egypt-Japan University of Science and Technology , Prof. Mohamed Abd El-Hady Ghazy - Institute of Basic and Applied Science, Egypt-Japan University of Science and Technology , Prof. Magdy Khalil Soliman - Faculty of Veterinary Meditie - Damanhour University , Prof. Alkhateib Yousry Gaafar - Veterinary Research Institute - National Research Centre<br/><br/><br/> |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | الكشف عن أمراض بكتيرية في أسماك المياه العذبة باستخدام تقنيات الميكروبيولوجيا و الأجسام المضادة وحيدة السلسلة : |
| Remainder of title | رسالة علمية مقدمة إلى المدرسة التخصصية للدرسات العليا العلوم الأساسية و التطبيقية : الجامعة المصرية اليابانية للعلوم و التكنولوجيا كاستيفاء جزئي لمتطلبات الحصول على درجة دكتوراه الفلسفة في البيوتكنولوجي / إعداد عبدالعاطي محمد عبدالعاطی صالح ; لجنة الإشراف على الرسالة أ . د أحمد لطفي عبدالموجود , أ . د محمد عبدالهادي غازی , أ . د علاء الدين عبد المعطي محمد عیسی , أ . م . د ساره اسامه سعد السيد ; لجنة المناقشة و الحكم على الرسالة أ . د أحمد لطفي عبدالموجود , أ . د محمد عبدالهادي غازی , ا . د مجدى خليل سلیمان , ا . د الخطيب يسرى جعفر<br/> |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Alexandria : |
| Name of publisher, distributor, etc. | Abdelatty Mohamed Abdelatty Saleh |
| Date of publication, distribution, etc. | 2025 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 109 leaves : |
| Other physical details | table ; |
| Dimensions | 30 cm |
| 500 ## - GENERAL NOTE | |
| General note | Includes a title page in Arabic |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) |
| Degree type | Doctoral |
| Name of granting institution | Egypt - Japan University of Science and Technology (E-JUST) - School of Basic and Applied Science - Biotechnology Department |
| Year degree granted | 2025 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Fish are a valuable food source due to their high nutritional value, palatability, and digestibility However, diseases exacerbated by intensive fish farming pose significan challenges High-density farming and pe margement often lead to chronic stres immension, and water commination, promoting bacterial outbreaks Key pathogens, such as Aeromonas hydrophila and Streptococcus agalactie, thrive in these environments, causing substantial losses in fish populations and potential threats to hum health Traditional methods of detecting bacterial pathogens, like culture-based techniques, are time- consuming and inadequate for identifying non-cultivable bacteria. Advanced techniques, such as 16S rRNA gene sequencing and immunoassays, erable more accurate identification and monitoring of pathogens The aquatic environment represents a potential source for transmitting A. hydrophila, which increase the mass mortalities in fish farms. This work aimed to determine the prevalence of A hydrophila in Ö. niloticus farms. Random samples, including water, sediment, and fish, were collected A total of 45 A. hydrophila isolates were presumptively identified using morph-chemical conventional tests A total of 42 A. Apdrophila isolates proved to be lipase producers Staphylococcus epidermidis has been identified as a causative agent of mass mortality in farmed O.milovics in Kafrelsheikh Governorate The collected diseased fish exhibited sever lesions in the hepatopancreas and eyes Isolates showed Gram-positive cocci characteristics and went unikt, congelamengutive, and murtutumdyä, un sirup likod ngan Tundu identification via 16S rRNA sequencing confirmed them as S. epidermidis. The four isolates in this study have similarities to other S. epidermidis isolates as follows : ASI strain of S epidermidis has a nucleotide sequence of 99.63%, similar to the reference isolate ; S. epidermidis sirain B7.Found in Shidal, India, A52 strain is 99.77% identical to the reference strain, LGC 305, which was isolated from the gut of a freshwater Indian loch ; while AS3 and AS4 strains are 99.86% and 99.99% identical to the reference strain found in Chinese fermented fish, respectively All eight isolates were subjected to an antibiogram test, and they were resistant to ampicillin, erythromycin, chloramphenicol, streptomycin, and amoxicillin however, they were slightly susceptible to tetracycline and were sensitive to gentamicin and vancomycin |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as a digital file (for more information please check our Digital Repository) |
| 590 ## - LOCAL NOTE (RLIN) | |
| Local note | AnD |
| Provenance (VM) [OBSOLETE] | ACM |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Abdel-Mawgood , Ahmed Lotfy , |
| Relator term | Supervisor |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ghazy , Mohamed Abd El-Hady , |
| Relator term | co-Supervisor |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Eissa , Alaa Eldin Abdel Mouty Mohamed , |
| Relator term | co-Supervisor |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | El-Sayed , Sarah Osama Saad , |
| Relator term | co-Supervisor |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Abdel-Mawgood , Ahmed Lotfy , |
| Relator term | Examinator |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ghazy , Mohamed Abd El-Hady , |
| Relator term | co-Examinator |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Soliman , Magdy Khalil , |
| Relator term | co-Examinator |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Gaafar , Alkhateib Yousry , |
| Relator term | co-Examinator |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | عبدالموجود , أحمد لطفي , |
| Relator term | مشرف |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | غازی , محمد عبدالهادي , |
| Relator term | مشرف مساعد |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | عیسی , علاء الدين عبد المعطي محمد , |
| Relator term | مشرف مساعد |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | السيد , ساره اسامه سعد , |
| Relator term | مشرف مساعد |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | عبدالموجود , أحمد لطفي , |
| Relator term | مناقش |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | غازی , محمد عبدالهادي , |
| Relator term | مناقش مساعد |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | سلیمان , مجدى خليل , |
| Relator term | مناقش مساعد |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | جعفر , الخطيب يسرى , |
| Relator term | مناقش مساعد |
| 901 ## - Cataloger information | |
| Cataloger Name | HaGeR |
| 902 ## - PDF File name | |
| PDF File name | EJUST_1302_ (2) |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Library of Congress Classification |
| Suppress in OPAC | No |
| Koha item type | Dissertations |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Home library | Current library | Date acquired | Total checkouts | Full call number | Barcode | Date last seen | Copy number | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Library of Congress Classification | Fayza Aboulnaga Central Library | مكتبة فايزة أبو النجا المركزية بالحرم الجامعي | Fayza Aboulnaga Central Library | مكتبة فايزة أبو النجا المركزية بالحرم الجامعي | 02/20/2025 | EPE PhD. 2025 01 | 10014206 | 02/20/2025 | C. 1 | 02/20/2025 | Dissertations |