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Detection of Bacterial Pathogen(s) in Freshwater Fish using Microbiological and Single Chain Fragment Antigen-Binding Region Techniques : A Thesis Submitted to the Graduate School of Basic and Applied Science : Egypt-Japan University of Science and Technology (E-JUST) : In Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy in Biotechnology / by Abdelatty Mohamed Abdelatty Saleh ; Supervisor Committee Prof. Ahmed Lotfy Abdel-Mawgood - Biotechnology Program, Institute of Basic and Applied Science, Egypt- Japan University of Science and Technology , Prof. Mohamed Abd El-Hady Ghazy - Biotechnology Program, Institute of Basic and Applied Science, Egypt- Japan University of Science and Technology , Prof. Alaa Eldin Abdel Mouty Mohamed Eissa - Department of Aquatic Animal Medicine and Management - Faculty of Veterinary Medicine - Cairo University , Associate Prof. Sarah Osama Saad El-Sayed - Oceanography Department - Faculty of Science - Alexandria University ; Examination Committee Prof. Ahmed Lotfy Abdel- Mawgood - Institute of Basic and Applied Science, Egypt-Japan University of Science and Technology , Prof. Mohamed Abd El-Hady Ghazy - Institute of Basic and Applied Science, Egypt-Japan University of Science and Technology , Prof. Magdy Khalil Soliman - Faculty of Veterinary Meditie - Damanhour University , Prof. Alkhateib Yousry Gaafar - Veterinary Research Institute - National Research Centre

By: Contributor(s): Material type: TextTextLanguage: English Summary language: Arabic Publication details: Alexandria : Abdelatty Mohamed Abdelatty Saleh 2025Description: 109 leaves : table ; 30 cmOther title:
  • الكشف عن أمراض بكتيرية في أسماك المياه العذبة باستخدام تقنيات الميكروبيولوجيا و الأجسام المضادة وحيدة السلسلة : رسالة علمية مقدمة إلى المدرسة التخصصية للدرسات العليا العلوم الأساسية و التطبيقية : الجامعة المصرية اليابانية للعلوم و التكنولوجيا كاستيفاء جزئي لمتطلبات الحصول على درجة دكتوراه الفلسفة في البيوتكنولوجي / إعداد عبدالعاطي محمد عبدالعاطی صالح ; لجنة الإشراف على الرسالة أ . د أحمد لطفي عبدالموجود , أ . د محمد عبدالهادي غازی , أ . د علاء الدين عبد المعطي محمد عیسی , أ . م . د ساره اسامه سعد السيد ; لجنة المناقشة و الحكم على الرسالة أ . د أحمد لطفي عبدالموجود , أ . د محمد عبدالهادي غازی , ا . د مجدى خليل سلیمان , ا . د الخطيب يسرى جعفر [Added title page title]
LOC classification:
  • EPE PhD. 2025  01
Issued also as a digital file (for more information please check our Digital Repository)Dissertation note: Thesis (Ph.D.) Doctoral Egypt - Japan University of Science and Technology (E-JUST) - School of Basic and Applied Science - Biotechnology Department 2025 Summary: Fish are a valuable food source due to their high nutritional value, palatability, and digestibility However, diseases exacerbated by intensive fish farming pose significan challenges High-density farming and pe margement often lead to chronic stres immension, and water commination, promoting bacterial outbreaks Key pathogens, such as Aeromonas hydrophila and Streptococcus agalactie, thrive in these environments, causing substantial losses in fish populations and potential threats to hum health Traditional methods of detecting bacterial pathogens, like culture-based techniques, are time- consuming and inadequate for identifying non-cultivable bacteria. Advanced techniques, such as 16S rRNA gene sequencing and immunoassays, erable more accurate identification and monitoring of pathogens The aquatic environment represents a potential source for transmitting A. hydrophila, which increase the mass mortalities in fish farms. This work aimed to determine the prevalence of A hydrophila in Ö. niloticus farms. Random samples, including water, sediment, and fish, were collected A total of 45 A. hydrophila isolates were presumptively identified using morph-chemical conventional tests A total of 42 A. Apdrophila isolates proved to be lipase producers Staphylococcus epidermidis has been identified as a causative agent of mass mortality in farmed O.milovics in Kafrelsheikh Governorate The collected diseased fish exhibited sever lesions in the hepatopancreas and eyes Isolates showed Gram-positive cocci characteristics and went unikt, congelamengutive, and murtutumdyä, un sirup likod ngan Tundu identification via 16S rRNA sequencing confirmed them as S. epidermidis. The four isolates in this study have similarities to other S. epidermidis isolates as follows : ASI strain of S epidermidis has a nucleotide sequence of 99.63%, similar to the reference isolate ; S. epidermidis sirain B7.Found in Shidal, India, A52 strain is 99.77% identical to the reference strain, LGC 305, which was isolated from the gut of a freshwater Indian loch ; while AS3 and AS4 strains are 99.86% and 99.99% identical to the reference strain found in Chinese fermented fish, respectively All eight isolates were subjected to an antibiogram test, and they were resistant to ampicillin, erythromycin, chloramphenicol, streptomycin, and amoxicillin however, they were slightly susceptible to tetracycline and were sensitive to gentamicin and vancomycin
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Dissertations Dissertations Fayza Aboulnaga Central Library | مكتبة فايزة أبو النجا المركزية بالحرم الجامعي EPE PhD. 2025 01 (Browse shelf(Opens below)) C. 1 Not for loan 10014206
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EPE MSc. 2024 05 Enhancing Grid Integration Through Control of Virtual Power Plant : A Thesis Submitted to the Graduate School of Electronics , Communication , and Computer Engineering (ECCE) : Egypt - Japan University of Science and Technology (E-JUST) : In Partial Fulfillment of the Requirements for the Degree of Master of Science in Electrical Power Engineering / EPE PhD. 2024 01 Optimal Energy Management of Renewable - Based Microgrids Considering Demand Response and Generation Uncertainty : A Thesis Submitted to the Graduate School of Electronic , Communication , and Computer Engineering (ECCE) : Egypt-Japan University of Science and Technology (E-JUST) : In Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy In Electric Power Engineering : EPE PhD. 2025 01 Performance Enhancement of Grid Connected Renewable Energy Systems During Faults : A Thesis Submitted to the Graduate School of Faculty of Engineering : Egypt-Japan University of Science and Technology (E-JUST) : In Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy in Electrical Power Engineering / EPE PhD. 2025 01 Detection of Bacterial Pathogen(s) in Freshwater Fish using Microbiological and Single Chain Fragment Antigen-Binding Region Techniques : A Thesis Submitted to the Graduate School of Basic and Applied Science : Egypt-Japan University of Science and Technology (E-JUST) : In Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy in Biotechnology / EPE PhD. 2025 01 Purification and Structure Elucidation of Secondary Metabolites Isolated from Certain Rhizospheric Bacteria and Assessing Their Pharmacological and Antimicrobial Activities / A Thesis Submitted to the Basic and Applied Science (BAS) Institute : Egypt-Japan University of Science and Technology (E-JUST) : In Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy (PhD) In Biotechnology / EPE PhD. 2025 01 Bioprospecting of Natural Products Associated with Microorganisms of Simmondsia chinensis : A Thesis Submitted to the Institute of Basic and Applied Science : Egypt-Japan University of Science and Technology (E-JUST) : In Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy In Biotechnology / EPE PhD. 2025 02 Fault Identification Schemes for Smart Grids with High PV Penetration Levels : A Thesis Submitted to the Graduate School of Electronics , Communications and Computer Engineering : Egypt-Japan University of Science and Technology (E-JUST) : In Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy in Electrical Power Engineering /

Includes a title page in Arabic

Thesis (Ph.D.) Doctoral Egypt - Japan University of Science and Technology (E-JUST) - School of Basic and Applied Science - Biotechnology Department 2025

Fish are a valuable food source due to their high nutritional value, palatability, and digestibility However, diseases exacerbated by intensive fish farming pose significan challenges High-density farming and pe margement often lead to chronic stres immension, and water commination, promoting bacterial outbreaks Key pathogens, such as Aeromonas hydrophila and Streptococcus agalactie, thrive in these environments, causing substantial losses in fish populations and potential threats to hum health Traditional methods of detecting bacterial pathogens, like culture-based techniques, are time- consuming and inadequate for identifying non-cultivable bacteria. Advanced techniques, such as 16S rRNA gene sequencing and immunoassays, erable more accurate identification and monitoring of pathogens The aquatic environment represents a potential source for transmitting A. hydrophila, which increase the mass mortalities in fish farms. This work aimed to determine the prevalence of A hydrophila in Ö. niloticus farms. Random samples, including water, sediment, and fish, were collected A total of 45 A. hydrophila isolates were presumptively identified using morph-chemical conventional tests A total of 42 A. Apdrophila isolates proved to be lipase producers Staphylococcus epidermidis has been identified as a causative agent of mass mortality in farmed O.milovics in Kafrelsheikh Governorate The collected diseased fish exhibited sever lesions in the hepatopancreas and eyes Isolates showed Gram-positive cocci characteristics and went unikt, congelamengutive, and murtutumdyä, un sirup likod ngan Tundu identification via 16S rRNA sequencing confirmed them as S. epidermidis. The four isolates in this study have similarities to other S. epidermidis isolates as follows : ASI strain of S epidermidis has a nucleotide sequence of 99.63%, similar to the reference isolate ; S. epidermidis sirain B7.Found in Shidal, India, A52 strain is 99.77% identical to the reference strain, LGC 305, which was isolated from the gut of a freshwater Indian loch ; while AS3 and AS4 strains are 99.86% and 99.99% identical to the reference strain found in Chinese fermented fish, respectively All eight isolates were subjected to an antibiogram test, and they were resistant to ampicillin, erythromycin, chloramphenicol, streptomycin, and amoxicillin however, they were slightly susceptible to tetracycline and were sensitive to gentamicin and vancomycin

Issued also as a digital file (for more information please check our Digital Repository)

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